Tissue dehydration and embedment
نویسنده
چکیده
Biological specimens contain up to 80% or more water which must be substituted in fixed cells (with the exception of special cryo-techniques) by some kind of embedding media to allow mechanical support for the sectioning process. Usually, embedding media cannot directly replace water, and fixation is followed by a sequence of dehydrating solutions from which the last must be miscible with the embedding matrix. The most employed dehydrating agents are ethanol and acetone.
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